A sample of a stool is submitted for laboratory analysis. There are several laboratory methods used to detect C. difficile toxin in the stool specimen. For many years, a cell cytotoxin assay has been used. This method incubates the stool specimen in the laboratory with tissue culture cells that are monitored over 24�- 48 hours for a specific reaction.

More recently, an enzyme immunoassay (EIA) to detect the toxins of C. difficile has been used. The EIA is faster, simpler to perform, and results are available in about an hour. However, it is slightly less sensitive than the cell culture method.

There are many ways to collect the samples. You can catch the stool on plastic wrap that is loosely placed over the toilet bowl and held in place by the toilet seat. Then put the sample in a clean container. One test kit supplies a special toilet tissue that you use to collect the sample.�After collecting the sample, you�put it in a clean container.

Do not mix urine, water, or toilet tissue with the sample.

For children wearing diapers, you can line the diaper with plastic wrap. If the plastic wrap is positioned properly, mixing of urine and stool can be prevented for a better sample.

There is no discomfort.

The test may be done when your doctor suspects that diarrhea is the result of recent antibiotic use. Antibiotics alter the bacterial flora in the colon ("helpful bacteria"), and this sometimes results in excessive growth of C. difficile and its toxins. Diarrhea caused by C. difficile following antibiotic use occurs frequently in hospitalized patients.

No C. difficile toxin detected.

Abnormal results mean that C. difficile toxins are likely present in the stool and are causing diarrhea.

There are no risks associated with testing for C. difficile toxin.

Since the test for C. difficile toxin is not 100% sensitive, several stool samples may be needed to detect it.